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Specifically, StableBit DrivePool and Windows doesn't need letters for the disks, nor even folder mount paths. These are there to make it easier for users to access the drives. But as somebody with 20+ drives, mounting the drives to folders makes things very easy. And we do have a guide on how to do so: https://wiki.covecube.com/StableBit_DrivePool_Q4822624

I have a similar setup on my backup NAS. All non-Drivepool drives are mounted to folders at c:\mount and I can access the drives directly from there if needed.

Windows only supports drive letters A through Z. However, it isn't necessary for a drive (other than the boot, system and pagefile drive, and perhaps CD/DVD drives and similar) to have a letter; drives can instead be accessed by mounting them as folders in another drive (e.g. C:\Array\Drive27, C:\Array\Drive28, etc) and furthermore itself DrivePool can have drives form part of a pool without being lettered or mounted at all. To add/remove drive letters or mount drives as folders in other drives, use Windows Disk Management: right-click a volume and click Change Drive Letters and Paths... Late edit for future readers: DON'T mount them as folders inside the pool drive itself, nor inside a poolpart folder. That risks a recursive loop, which would be bad.