Go to My Computer, or My PC Icon, if you have the icons turned on, on your Desktop Screen. Right click on it and select more option. And click on manage.
In the Computer Management Window you will see Disk Management. Click on that and all your hard drives will show up. Select one of the drives and right click.
You will see Change Drive Letter and Paths. Click on that. Select remove. You will get a warning. But that's ok if the hard drive is only for storing data. (Videos, documents and pictures. Stuff like that).
No programs installed on the drives or looking for a drive letter to work. ( Just Data Storage. StableBit will still work. It does not use the drive letters to function properly).
If later you need to access that drive or drives outside of the storage pool. Just do the same thing and click add instead of remove the drive letter.