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They stay on the disk, outside of the pool, and are considered "other" data. And nothing will be in the pool. You want this: http://wiki.covecube.com/StableBit_DrivePool_Q4142489 As for the existing drive letters, you may want to take a look at this: http://wiki.covecube.com/StableBit_DrivePool_Q4822624

Editing my post for a bit more clarity: You can use duplication and then simply take the drive out of the machine, and revert duplication to x1. However according to the DrivePool feature list, when you remove a disk from the pool via the software options: Provided you have enough available space on other disks in the pool, then Christopher is correct in that a removal will transfer all files to other disks prior to removing the drive you wanted. I'm not sure which option is faster. Either should work. Christopher's advice is probably the best you're going to get.